Hyphae-on-a-Chip - A microfluidic platform for the study of protrusive forces in hyphal invasion

Status: Current

Dates: 2016 - 2019

Māori Relevant Content: Yes

Funding:

  • Royal Society of New Zealand (Marsden Fund, 2016 - 2019)

Project Abstract

Fungi and oomycetes grow as pathogenic species on both plants and animals. They can have significant effects on human health and affairs, either directly through infections or indirectly through loss of crop and other species. The ability to grow invasively is one of the key processes in the pathogenicity of these organisms. Protrusive forces generated by the tip of a growing hyphae aid in the invasion. The aim of this project is to develop a platform of Lab-on-a-chip devices based around arrays of force sensing micropillars, which will help to extend the understanding of the mechanisms that underlie invasive growth. This is likely to involve an interplay of enzymatic breakdown of host tissue and a protrusive force generated by the tip of the growing hypha. The protrusive force will be influenced by two factors: the turgor pressure of the hypha and the yielding capacity of the tip. The measurement of this protrusive force and determination of the factors that underlie it are the main aims of this proposal. Be

Researchers - UC Staff

Researchers - Non-UC Staff

  • Yiling Sun: Postgraduate student; University of Canterbury

Associated Groups

Subject Area: Disciplines