Evidence is accumulating that the cells’ physical micro- and nano-environments are crucial. In this proposal, we will apply, for the first time, measurable forces on groups of cells in 3D clusters using microfluidic channels with flexible actuators and force sensing arrays. The influence of the applied physical forces on ovarian cancer cell growth and spread will be quantified. The biological activities of the cells under force will be monitored. We expect to establish that forces exerted and experienced within small areas of tissues may stimulate or inhibit tumour growth, and conversely modification of those forces could induce tumour regression.