Research/Scholarly/Creative Works

• Allison TM., Degiacomi MT., Marklund EG., Jovine L., Elofsson A., Benesch JLP. and Landreh M. (2022) Complementing machine learning-based structure predictions with native mass spectrometry. Protein Science 31(6) http://dx.doi.org/10.1002/pro.4333. (Journal Articles)

• Given FM., Moran F., Johns AS., Titterington JA., Allison TM., Crittenden DL., Johnston JM. and Tsai FT. (2022) The structure of His-tagged Geobacillus stearothermophilus purine nucleoside phosphorylase reveals a `spanner in the works'. Acta crystallographica. Section F, Structural biology communications 78: 416-422. http://dx.doi.org/10.1107/S2053230X22011025. (Journal Articles)

• Yen HY., Abramsson ML., Agasid MT., Lama D., Gault J., Liko I., Kaldmäe M., Saluri M., Qureshi AA. and Suades A. (2022) Electrospray ionization of native membrane proteins proceeds via a charge equilibration step. RSC Advances 12(16): 9671-9680. http://dx.doi.org/10.1039/d2ra01282k. (Journal Articles)

• Abramsson ML., Sahin C., Hopper JTS., Branca RMM., Danielsson J., Xu M., Chandler SA., Österlund N., Ilag LL. and Leppert A. (2021) Charge Engineering Reveals the Roles of Ionizable Side Chains in Electrospray Ionization Mass Spectrometry. JACS Au 1(12): 2385-2393. http://dx.doi.org/10.1021/jacsau.1c00458. (Journal Articles)

• Sahin C., Österlund N., Leppert A., Johansson J., Marklund EG., Benesch JLP., Ilag LL., Allison TM. and Landreh M. (2021) Ion mobility-mass spectrometry shows stepwise protein unfolding under alkaline conditions. Chemical Communications 57(12): 1450-1453. http://dx.doi.org/10.1039/d0cc08135c. (Journal Articles)

• Allison TM. and Agasid MT. (2020) Native Protein Mass Spectrometry. Methods in Molecular Biology: 287-299. http://dx.doi.org/10.1007/978-1-4939-9869-2_15. (Chapters)

• Allison TM., Barran P., Benesch JLP., Cianferani S., Degiacomi MT., Gabelica V., Grandori R., Marklund EG., Menneteau T. and Migas LG. (2020) Software Requirements for the Analysis and Interpretation of Native Ion Mobility Mass Spectrometry Data. Analytical Chemistry 92(16): 10881-10890. http://dx.doi.org/10.1021/acs.analchem.9b05792. (Journal Articles)

• Allison TM., Barran P., Cianférani S., Degiacomi MT., Gabelica V., Grandori R., Marklund EG., Menneteau T., Migas LG. and Politis A. (2020) Computational Strategies and Challenges for Using Native Ion Mobility Mass Spectrometry in Biophysics and Structural Biology. Analytical Chemistry 92(16): 10872-10880. http://dx.doi.org/10.1021/acs.analchem.9b05791. (Journal Articles)

• Bolla JR., Corey RA., Sahin C., Gault J., Hummer A., Hopper JTS., Lane DP., Drew D., Allison TM. and Stansfeld PJ. (2020) A Mass-Spectrometry-Based Approach to Distinguish Annular and Specific Lipid Binding to Membrane Proteins. Angewandte Chemie - International Edition 59(9): 3523-3528. http://dx.doi.org/10.1002/anie.201914411. (Journal Articles)

• Landreh M., Sahin C., Gault J., Sadeghi S., Drum CL., Uzdavinys P., Drew D., Allison T., Degiacomi M. and Marklund E. (2020) Predicting the Shapes of Protein Complexes Through Collision Cross Section Measurements and Database Searches. ChemRxiv http://dx.doi.org/10.26434/chemrxiv.12275057.v1. (Journal Articles)

• Landreh M., Sahin C., Gault J., Sadeghi S., Drum CL., Uzdavinys P., Drew D., Allison TM., Degiacomi MT. and Marklund EG. (2020) Predicting the Shapes of Protein Complexes through Collision Cross Section Measurements and Database Searches. Analytical Chemistry 92(18): 12297-12303. http://dx.doi.org/10.1021/acs.analchem.0c01940. (Journal Articles)

• Allison T., Barran P., Benesch J., Cianferani S., Degiacomi M., Gabelica V., Grandori R., Marklund E., Menneteau T. and Migas L. (2019) Software for the analysis and interpretation of native mass spectrometry data. ChemRxiv http://dx.doi.org/10.26434/chemrxiv.11440254.v1. (Journal Articles)

• Allison T., Barran P., Cianferani S., Degiacomi M., Gabelica V., Grandori R., Marklund E., Mennteau T., Migas L. and Politis A. (2019) Computational strategies and challenges for using native ion mobility mass spectrometry in biophysics and structural biology. ChemRxiv http://dx.doi.org/10.26434/chemrxiv.11439558.v1. (Journal Articles)

• Allison TM. and Bechara C. (2019) Structural mass spectrometry comes of age: New insight into protein structure, function and interactions. Biochemical Society Transactions 47(1): 317-327. http://dx.doi.org/10.1042/BST20180356. (Journal Articles)

• Allison TM. and Landreh M. (2019) Ion Mobility in Structural Biology. In Donald WA; Prell JS (Ed.), Comprehensive Analytical Chemistry: 161-195. http://dx.doi.org/10.1016/bs.coac.2018.10.001. (Chapters)

• Collier MP., Reid Alderson T., De Villiers CP., Nicholls D., Gastall HY., Allison TM., Degiacomi MT., Jiang H., Mlynek G. and Fürst DO. (2019) HspB1 phosphorylation regulates its intramolecular dynamics and mechanosensitive molecular chaperone interaction with filamin C. Science Advances 5(5) http://dx.doi.org/10.1126/sciadv.aav8421. (Journal Articles)

• Kaldmäe M., Österlund N., Lianoudaki D., Sahin C., Bergman P., Nyman T., Kronqvist N., Ilag LL., Allison TM. and Marklund EG. (2019) Gas-Phase Collisions with Trimethylamine-N-Oxide Enable Activation-Controlled Protein Ion Charge Reduction. Journal of the American Society for Mass Spectrometry 30(8): 1385-1388. http://dx.doi.org/10.1007/s13361-019-02177-8. (Journal Articles)

• Bolla JR., Sauer JB., Wu D., Mehmood S., Allison TM. and Robinson CV. (2018) Direct observation of the influence of cardiolipin and antibiotics on lipid II binding to MurJ. Nature Chemistry 10(3): 363-371. http://dx.doi.org/10.1038/nchem.2919. (Journal Articles)

• Gault J., Lianoudaki D., Kaldmäe M., Kronqvist N., Rising A., Johansson J., Lohkamp B., Laín S., Allison TM. and Lane DP. (2018) Mass Spectrometry Reveals the Direct Action of a Chemical Chaperone. Journal of Physical Chemistry Letters 9(14): 4082-4086. http://dx.doi.org/10.1021/acs.jpclett.8b01817. (Journal Articles)

• Hochberg GKA., Shepherd DA., Marklund EG., Santhanagoplan I., Degiacomi MT., Laganowsky A., Allison TM., Basha E., Marty MT. and Galpin MR. (2018) Structural principles that enable oligomeric small heat-shock protein paralogs to evolve distinct functions. Science 359(6378): 930-935. http://dx.doi.org/10.1126/science.aam7229. (Journal Articles)

• Liko I., Degiacomi MT., Lee S., Newport TD., Gault J., Reading E., Hopper JTS., Housden NG., White P. and Colledge M. (2018) Lipid binding attenuates channel closure of the outer membrane protein OmpF. Proceedings of the National Academy of Sciences of the United States of America 115(26): 6691-6696. http://dx.doi.org/10.1073/pnas.1721152115. (Journal Articles)

• Yewdall NA., Allison TM., Pearce FG., Robinson CV. and Gerrard JA. (2018) Self-assembly of toroidal proteins explored using native mass spectrometry. Chemical Science 9(28): 6099-6106. http://dx.doi.org/10.1039/c8sc01379a. (Journal Articles)

• Hopper JTS., Ambrose S., Grant OC., Krumm SA., Allison TM., Degiacomi MT., Tully MD., Pritchard LK., Ozorowski G. and Ward AB. (2017) The Tetrameric Plant Lectin BanLec Neutralizes HIV through Bidentate Binding to Specific Viral Glycans. Structure 25(5): 773-782.e5. http://dx.doi.org/10.1016/j.str.2017.03.015. (Journal Articles)

• Yen HY., Hopper JTS., Liko I., Allison TM., Zhu Y., Wang D., Stegmann M., Mohammed S., Wu B. and Robinson CV. (2017) Ligand binding to a G protein–coupled receptor captured in a mass spectrometer. Science Advances 3(6) e1701016: 6. http://dx.doi.org/10.1126/sciadv.1701016. (Journal Articles)

• Allison TM., Landreh M., Benesch JLP. and Robinson CV. (2016) Low Charge and Reduced Mobility of Membrane Protein Complexes Has Implications for Calibration of Collision Cross Section Measurements. Analytical Chemistry 88(11): 5879-5884. http://dx.doi.org/10.1021/acs.analchem.6b00691. (Journal Articles)

• Liko I., Allison TM., Hopper JT. and Robinson CV. (2016) Mass spectrometry guided structural biology. Current Opinion in Structural Biology 40: 136-144. http://dx.doi.org/10.1016/j.sbi.2016.09.008. (Journal Articles)

• Liko I., Hopper JTS., Allison TM., Benesch JLP. and Robinson CV. (2016) Negative Ions Enhance Survival of Membrane Protein Complexes. Journal of the American Society for Mass Spectrometry 27(6): 1099-1104. http://dx.doi.org/10.1007/s13361-016-1381-5. (Journal Articles)

• Nazmi AR., Lang EJM., Bai Y., Allison TM., Othman MH., Panjikar S., Arcus VL. and Parker EJ. (2016) Interdomain conformational changes provide allosteric regulation en route to chorismate. Journal of Biological Chemistry 291(42): 21836-21847. http://dx.doi.org/10.1074/jbc.M116.741637. (Journal Articles)

• Podobnik M., Savory P., Rojko N., Kisovec M., Wood N., Hambley R., Pugh J., Wallace EJ., McNeill L. and Bruce M. (2016) Crystal structure of an invertebrate cytolysin pore reveals unique properties and mechanism of assembly. Nature Communications 7 11598 http://dx.doi.org/10.1038/ncomms11598. (Journal Articles)

• Allison TM., Reading E., Liko I., Baldwin AJ., Laganowsky A. and Robinson CV. (2015) Quantifying the stabilizing effects of protein-ligand interactions in the gas phase. Nature Communications 6 http://dx.doi.org/10.1038/ncomms9551. (Journal Articles)

• Mehmood S., Allison TM. and Robinson CV. (2015) Mass spectrometry of protein complexes: From origins to applications. Annual Review of Physical Chemistry 66: 453-474. http://dx.doi.org/10.1146/annurev-physchem-040214-121732. (Journal Articles)

• Reading E., Liko I., Allison TM., Benesch JLP., Laganowsky A. and Robinson CV. (2015) The Role of the Detergent Micelle in Preserving the Structure of Membrane Proteins in the Gas Phase. Angewandte Chemie - International Edition 54(15): 4577-4581. http://dx.doi.org/10.1002/anie.201411622. (Journal Articles)

• Laganowsky A., Reading E., Allison TM., Ulmschneider MB., Degiacomi MT., Baldwin AJ. and Robinson CV. (2014) Membrane proteins bind lipids selectively to modulate their structure and function. Nature 510(7503): 172-175. http://dx.doi.org/10.1038/nature13419. (Journal Articles)

• Mehmood S., Marcoux J., Hopper JTS., Allison TM., Liko I., Borysik AJ. and Robinson CV. (2014) Charge reduction stabilizes intact membrane protein complexes for mass spectrometry. Journal of the American Chemical Society 136(49): 17010-17012. http://dx.doi.org/10.1021/ja510283g. (Journal Articles)

• Allison TM., Cochrane FC., Jameson GB. and Parker EJ. (2013) Examining the role of intersubunit contacts in catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase. Biochemistry 52(27): 4676-4686. http://dx.doi.org/10.1021/bi400521f. (Journal Articles)

• Cross PJ., Allison TM., Dobson RCJ., Jameson GB. and Parker EJ. (2013) Engineering allosteric control to an unregulated enzyme by transfer of a regulatory domain. Proceedings of the National Academy of Sciences of the United States of America 110(6): 2111-2116. http://dx.doi.org/10.1073/pnas.1217923110. (Journal Articles)

• Cross PJ., Pietersma AL., Allison TM., Wilson-Coutts SM., Cochrane FC. and Parker EJ. (2013) Neisseria meningitidis expresses a single 3-deoxy-D-arabino heptulosonate 7-phosphate synthase that is inhibited primarily by phenylalanine. Protein Science 22: 1087-1099. http://dx.doi.org/10.1002/pro.2293. (Journal Articles)

• Allison TM. (2012) Substrate specificity and mutational studies of KD08PS. Christchurch, New Zealand. University of Canterbury. (Theses / Dissertations)

• Allison TM., Hutton RD., Cochrane FC., Yeoman JA., Jameson GB. and Parker EJ. (2011) Targeting the role of a key conserved motif for substrate selection and catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase. Biochemistry 50(18): 3686-3695. http://dx.doi.org/10.1021/bi200251f. (Journal Articles)

• Allison TM., Hutton RD., Jiao W., Gloyne BJ., Nimmo EB., Jameson GB. and Parker EJ. (2011) An extended β7α7 substrate-binding loop is essential for efficient catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase. Biochemistry 50(43): 9318-9327. http://dx.doi.org/10.1021/bi201231e. (Journal Articles)

• Allison TM., Yeoman JA., Hutton RD., Cochrane FC., Jameson GB. and Parker EJ. (2010) Specificity and mutational analysis of the metal-dependent 3-deoxy-D-manno-octulosonate 8-phosphate synthase from Acidithiobacillus ferrooxidans. Biochimica Et Biophysica Acta (BBA) - Proteins & Proteomics 1804(7): 1526-1536. http://dx.doi.org/10.1016/j.bbapap.2010.04.005. (Journal Articles)

Future Research

• N-linked glycosylation
• Native mass spectrometry mechanisms